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Re: Enzyme-Linked Immunosorbent Assay

Posted by Deneb on April 10, 2006, at 22:00:05

In reply to Re: Enzyme-Linked Immunosorbent Assay » Deneb, posted by Phillipa on April 10, 2006, at 21:21:52

The Western Blot is a technique. It's the transfer of the results of an RNA electrophoresis onto a membrane. It's like a Northern Blot, but with RNA. It can be used to check for specific RNAs.

There is also something called a Northern Blot which I'm more familiar with, that involves DNA. You chop up DNA with restriction endonucleases and electrophoresis them. Then you use a Northern Blot to transfer the pattern of DNA migration onto a membrane. You visualize the bands of DNA using DNA probes with markers.

Deneb*


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