Psycho-Babble Social Thread 630699

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Re: Just had the police at the door... NikkiT2

Posted by Racer on April 9, 2006, at 12:53:59

In reply to Re: Just had the police at the door... Racer, posted by NikkiT2 on April 9, 2006, at 5:20:23

>
> As an slight aside.. Clubs in Amsterdam used to have a little guy with a chemistry set of some sort that would test ecstasy pills and tell you roughly what dodgies they contained and how much MDMA they contained!
>
> Nikki

THey had similar things around here, folks who'd use that sort of ELISA test to check what you had. I never saw that, after my time in the clubs, but I read about it.

I look back, and am amazed I havea brain at all... I took a lot of stupid things in the day...

xoxo

 

Re: Just had the police at the door... Racer

Posted by Phillipa on April 9, 2006, at 21:20:41

In reply to Re: Just had the police at the door... NikkiT2, posted by Racer on April 9, 2006, at 12:53:59

Elisa test and the Western blot are used to test for lymes's Disease the Western blot is the only reliable one. Love Phillipa

 

I guess I'm confused Phillipa

Posted by Racer on April 9, 2006, at 21:55:55

In reply to Re: Just had the police at the door... Racer, posted by Phillipa on April 9, 2006, at 21:20:41

> Elisa test and the Western blot are used to test for lymes's Disease the Western blot is the only reliable one. Love Phillipa


What did my post have to do with Lyme's Disease?

 

Re: Just had the police at the door... Racer

Posted by Phillipa on April 9, 2006, at 22:06:16

In reply to Re: Just had the police at the door... NikkiT2, posted by Racer on April 9, 2006, at 12:53:59

Nothing that was my point the Elisa test is used to diagnose lynes along with the Western Blot. Just thought you could have been confused about the name of the test. Since I have never taken illegal meds I have no clue how they test for them. I only know the unrine and blood tests for alchohol and illegal substances when you are drug tested for a job. Love Phillipa

 

Enzyme-Linked Immunosorbent Assay Phillipa

Posted by Racer on April 10, 2006, at 1:21:45

In reply to Re: Just had the police at the door... Racer, posted by Phillipa on April 9, 2006, at 22:06:16

> Nothing that was my point the Elisa test is used to diagnose lynes along with the Western Blot. Just thought you could have been confused about the name of the test. Since I have never taken illegal meds I have no clue how they test for them. I only know the unrine and blood tests for alchohol and illegal substances when you are drug tested for a job. Love Phillipa

ELISA is a kind of test. There are ELISA tests for many different things, from blood type to HIV to Lyme's to certain illegal drugs. It's not a specific test for any one thing, but a method of testing.

 

Re: I don't know what dodgy means either (nm) NikkiT2

Posted by Joan797 on April 10, 2006, at 8:10:23

In reply to I think I made a word up! Joan797, posted by NikkiT2 on April 9, 2006, at 8:55:47

 

Re: A Roofy? Phillipa

Posted by Joan797 on April 10, 2006, at 8:12:13

In reply to Re: Just had the police at the door... Joan797, posted by Phillipa on April 9, 2006, at 12:08:01

That's the only other word I've ever heard for date rape drug. I got that word from the ya-ya sisterhood movie, that of course I watched so many times I broke the tape but can still quote many many lines from.

 

Re: Just had the police at the door... Racer

Posted by Poet on April 10, 2006, at 13:44:04

In reply to Just had the police at the door..., posted by Racer on April 8, 2006, at 19:51:13

Hi Racer,

When he was a teenager my husband got yelled at by his mother for keeping LSD in the freezer. It wasn't his- it was his older brother's, who now has a PhD in pharmacology. ROFL.

Poet

 

Dodgy means..... Joan797

Posted by Meri-Tuuli on April 10, 2006, at 14:52:01

In reply to Re: I don't know what dodgy means either (nm) NikkiT2, posted by Joan797 on April 10, 2006, at 8:10:23

We definately need an English - American dictionary.

Well dodgy is a really common word here in the UK.

From wikipedia dictionary:

"Derived from the verb dodge. First used in mid-19th century England.

Adjective

dodgy (UK)

evasive and shifty
Asked why, a spokesman gave a dodgy answer about legal ramifications.

unsound and unreliable
Never listen to dodgy advice.

The dodgy old machine kept breaking down.

dishonest
The more money the better, because there is always that dodgy politician or corrupt official to bribe.

I am sure you wouldn't want to be seen buying dodgy gear, would you? (stolen goods).

risky
This is a slightly dodgy plan, because there is a lot that is being changed for this fix.

deviant
He's a dodgy Peeping Tom.

weird
The situation was right dodgy.

I'm feeling dodgy today, probably got the flu."

 

Re: Enzyme-Linked Immunosorbent Assay Racer

Posted by Phillipa on April 10, 2006, at 19:43:59

In reply to Enzyme-Linked Immunosorbent Assay Phillipa, posted by Racer on April 10, 2006, at 1:21:45

According to google it is used to pick up any infectious disease including HIV from illegal drug use by dirty needles and blood leading to hepatitis C. It doesn't pick up an actual drug you may have used. Just the result of and it is a very inaccurated test. Love Phillipa Oh and very often lymes needs much further testing as it is positive for syphylis which of course I had to be further testing for hence the Western Blot and a bunch of other ones Igg etc.

 

Re: Enzyme-Linked Immunosorbent Assay

Posted by Deneb on April 10, 2006, at 20:05:48

In reply to Enzyme-Linked Immunosorbent Assay Phillipa, posted by Racer on April 10, 2006, at 1:21:45

> ELISA is a kind of test. There are ELISA tests for many different things, from blood type to HIV to Lyme's to certain illegal drugs. It's not a specific test for any one thing, but a method of testing.

I've done an ELISA before, in my cell bio lab.

I dropped that class though, so I don't remember the details.

Deneb

 

Re: Enzyme-Linked Immunosorbent Assay

Posted by Deneb on April 10, 2006, at 20:09:43

In reply to Re: Enzyme-Linked Immunosorbent Assay, posted by Deneb on April 10, 2006, at 20:05:48

I remember antibodies are involved. Antibodies that are absorbed onto this plastic plate with wells.

I'm such a bad student...

Deneb

 

Re: Enzyme-Linked Immunosorbent Assay Deneb

Posted by Racer on April 10, 2006, at 20:52:28

In reply to Re: Enzyme-Linked Immunosorbent Assay, posted by Deneb on April 10, 2006, at 20:09:43

>
> I'm such a bad student...
>
> Deneb

It's not about being a bad student, Deneb. It's about not remembering something that really isn't applicable to you. Selective inattention or selective retention.

And frankly, I only remember the very basics of it.

And because I can't resist the urge to offer advice: check out the way you describe yourself. In the past 24 hours or so, you've told me you don't have my brains and that you are a bad student. There's nothing wrong with your brain, and you could be a great student if you chose to be, but as long as you continue to spin yourself so negatively, you're gonna be wasting energy. How about this, just as an experiment: for one week, speak of yourself as you would speak of someone else, for whom you felt respect and affection. Even if you don't quite believe it, even if it feels as though you're bragging, speak of yourself as if you liked you. Even when you're talking to yourself. Just -- give it a try. Maybe you'll find you feel better.

And please take all this in the spirit in which it is offered, you know?

 

Re: Enzyme-Linked Immunosorbent Assay Deneb

Posted by Phillipa on April 10, 2006, at 21:21:52

In reply to Re: Enzyme-Linked Immunosorbent Assay, posted by Deneb on April 10, 2006, at 20:05:48

Deneb could you maybe look up the actural definition of the Elisa test? Google there are just too many sites and they all refer to infection. Aids, lymes, hepatitis C. I know it's not acurate for a diagnosis for lymes but what will it tell about other diseases and druges you may have used. Is it specific or does only tell you've either been exposed or have used a specific drug. I know the Western Blot is for Lymes and says whether you have ever had it and it will always be positive if you've had the disease. the others are beyond my knowledge. Love Phillipa

 

Re: Enzyme-Linked Immunosorbent Assay

Posted by Deneb on April 10, 2006, at 22:00:05

In reply to Re: Enzyme-Linked Immunosorbent Assay Deneb, posted by Phillipa on April 10, 2006, at 21:21:52

The Western Blot is a technique. It's the transfer of the results of an RNA electrophoresis onto a membrane. It's like a Northern Blot, but with RNA. It can be used to check for specific RNAs.

There is also something called a Northern Blot which I'm more familiar with, that involves DNA. You chop up DNA with restriction endonucleases and electrophoresis them. Then you use a Northern Blot to transfer the pattern of DNA migration onto a membrane. You visualize the bands of DNA using DNA probes with markers.

Deneb*

 

Re: Enzyme-Linked Immunosorbent Assay

Posted by Deneb on April 10, 2006, at 22:06:09

In reply to Re: Enzyme-Linked Immunosorbent Assay, posted by Deneb on April 10, 2006, at 22:00:05

Sorry, I wasn't really explaining things too well in my last post. I should have explained things like electrophoresis, but I didn't.

In electrophoresis, you make this gel, with little wells at the top. Then you fill each well with a different mixture of either chopped up RNAs or DNAs. Then you run an electric current through the gel and the bands of DNA or RNA migrate through the gel. Bands are separated out according to the length of the segments of RNA or DNA.

Deneb*

 

Re: Enzyme-Linked Immunosorbent Assay Deneb

Posted by Phillipa on April 10, 2006, at 22:06:50

In reply to Re: Enzyme-Linked Immunosorbent Assay, posted by Deneb on April 10, 2006, at 22:00:05

Deneb you are truly brillient!!!!!!! Tell me more. I think you should be a scientist and find a cure for some aweful disease. You can do it I know you can. Love Phillipa

 

Wow! Deneb

Posted by gardenergirl on April 10, 2006, at 22:17:30

In reply to Re: Enzyme-Linked Immunosorbent Assay, posted by Deneb on April 10, 2006, at 22:00:05

See, you are so not brainless. :)

gg

 

So, getting back to the original topic... Racer

Posted by gardenergirl on April 10, 2006, at 22:18:50

In reply to Just had the police at the door..., posted by Racer on April 8, 2006, at 19:51:13

Good for you for calling the authorities. Whatever it was, it's not something I'd want in the hands of children.

gg

 

Re: Enzyme-Linked Immunosorbent Assay Deneb

Posted by Phillipa on April 10, 2006, at 22:46:18

In reply to Re: Enzyme-Linked Immunosorbent Assay, posted by Deneb on April 10, 2006, at 22:06:09

Deneb so that's what the bands mean. Supposedly the number of bands tells how bad the lymes is. You can E-mail me more of this info if gg wants to get back to the original topic. Love jan

 

Re: Enzyme-Linked Immunosorbent Assay: correction

Posted by Deneb on April 11, 2006, at 6:43:26

In reply to Re: Enzyme-Linked Immunosorbent Assay, posted by Deneb on April 10, 2006, at 22:00:05

Argh! This just proves how stupid I am.

A Western blot is for proteins. A Northern blot is for RNA and a Southern blot is for DNA.

I previously wrote down that a Western was for RNA and a Northern is for DNA.

I suddenly woke up this morning and that's the first thing that popped into my head.

Deneb*

 

Re: Enzyme-Linked Immunosorbent Assay - actually Deneb

Posted by madeline on April 11, 2006, at 10:18:57

In reply to Re: Enzyme-Linked Immunosorbent Assay, posted by Deneb on April 10, 2006, at 22:00:05

A western blot detects proteins immobilized on a membrane.

A southern blot detects DNA

A northern blot detects RNA.

Electrophoresis is a simple technique of separating a mixture of things based on a characteristic of the protein.

Trust me. I have a Ph.D. in this crap.

 

Re: Enzyme-Linked Immunosorbent Assay - actually madeline

Posted by Deneb on April 11, 2006, at 12:21:46

In reply to Re: Enzyme-Linked Immunosorbent Assay - actually Deneb, posted by madeline on April 11, 2006, at 10:18:57

> A western blot detects proteins immobilized on a membrane.
>
> A southern blot detects DNA
>
> A northern blot detects RNA.
>
> Electrophoresis is a simple technique of separating a mixture of things based on a characteristic of the protein.

Electrophoresis also separates out mixtures of DNA and RNA, right?

>
> Trust me. I have a Ph.D. in this crap.

Why do you think it's crap?

Deneb*

 

Re: Enzyme-Linked Immunosorbent Assay - actually Deneb

Posted by madeline on April 11, 2006, at 23:18:05

In reply to Re: Enzyme-Linked Immunosorbent Assay - actually madeline, posted by Deneb on April 11, 2006, at 12:21:46

electrophoresis can sort out a mixture of just about anything.

It's crap (and I use that term loosely) because I work with them everyday.

They are just tools used to answer questions.

And they don't always work. A western blot, in principle, should be the simplest thing in the world to do - but it isn't. It can take months to get usable data from one.

Northerns are just about impossible and southerns are nearly as bad.

Have you ever heard of a "Far western" blot? That one is kinda neat.

 

Re: Enzyme-Linked Immunosorbent Assay - actually madeline

Posted by Deneb on April 12, 2006, at 0:04:07

In reply to Re: Enzyme-Linked Immunosorbent Assay - actually Deneb, posted by madeline on April 11, 2006, at 23:18:05

> It's crap (and I use that term loosely) because I work with them everyday.

Cool. What do you do?

> They are just tools used to answer questions.
>
> And they don't always work. A western blot, in principle, should be the simplest thing in the world to do - but it isn't. It can take months to get usable data from one.
>
> Northerns are just about impossible and southerns are nearly as bad.

Wow, I didn't know that. I've only done one Western before. Everything seems so simple on paper. LOL

>
> Have you ever heard of a "Far western" blot? That one is kinda neat.

Nope. What is it?

Deneb*


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