Posted by Larry Hoover on November 8, 2004, at 11:09:03
In reply to Re: dopamine oxidation » Larry Hoover, posted by raybakes on November 8, 2004, at 3:16:07
> > > I can only tell you what if feels like - I think high dose ascorbate would be fine with someone with normal health. I know if I take over 1 gramme of magensium ascorbate, I feel incredibly ill, mentally.
> >
> > Maybe that's the magnesium "talking"?
>
> Don't think so, feel great on magnesium malate/citrate.Okay. <shrug>
> > I really doubt there's a pH mechanism at play. There are many many ways for cells to adjust pH. There are substantial buffer systems in place, just for that purpose. Do you understand the chemistry of buffer systems?
>
> I don't understand all the buffer systems but suspect the bicarbonate buffering to be underfunctioning.That would be quite a stretch. Every breath you take is part of a feedback loop to the partial pressure of carbon dioxide in the blood.
Here's a good overview.
http://www.cvm.okstate.edu/courses/vmed5412/14%20Acid-Base.docWithin cells, the bicarbonate buffer is replaced by protein/amino acid buffers, and phosphate buffering. The latter buffer is what makes those lime scale/iron stain removers powerful enough to remove deposits on e.g. bathroom fixtures, but moderate enough that you can get them on your hands without fear.
> If I raise carbon dioxide by holding my breath, or drinking carbonated water, my brain becomes more clear. Might be something to do with the bohr effect too?
Almost certainly it is the Bohr effect. Not much more than a parlor trick, though, as you will breath again and restore the normal chemistry.
> Also I think it's common in autoimmune disease to have antibodies to carbonic anhydrase, vital for bicarbonate buffering and digestive function.
It seems to have a vital role in the transfer of oxygen to and from hemoglobin, among other effects. Carbonic anhydrase, in the presence of CO2, will facilitate the "unloading" of O2 from hemoglobin, while simultaneously replacing the O2 with H+ and bicarbonate. Each of the latter will then interact with the aqueous component of blood.
> Bicarbonate seems to have both positive and negative effects on peroxynitrite - some abstracts say it helps detoxify it, some say it hinders uric acid, ascorbate, GSH in it's detoxification.
Yes, but....
Bicarbonate is everywhere in your circulation, so whether it tips this balance a certain way, and that one another, is moot.
>
> Found this abstract on how carbonic anhydrase is invovled in helping produce the cerebrospinal fluid, and it thought to influence 'neuroexcitation'.
>
> 'Carbonic anhydrase IV on brain capillary endothelial cells: a marker associated with the blood-brain barrier.'
>
>
> http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=1495971&dopt=AbstractWhen I read that, I see that a specific form of carbonic anhydrase is responsible for facilitating gas transfer at the capillary wall in the brain, i.e. at the blood-brain barrier. Red blood cells can't get across, so the unloading occurs at this membrane surface. Diffusion probably takes the 02 to the mitochondria.
> Still think buffering is really important - especially as in fatigued cells, the mitochondria fail to get sufficient numbers of protons combining with oxygen to maintain an even pH.
>
> RayOnly in extreme circumstances. That's what buffers do....maintain supplies of H+ without changing pH dramatically.
http://www.sparknotes.com/chemistry/acidsbases/buffers/section1.html
Lar
poster:Larry Hoover
thread:404137
URL: http://www.dr-bob.org/babble/alter/20041108/msgs/413265.html