Posted by Larry Hoover on July 31, 2004, at 10:57:43
In reply to is ginkgo neurotrophic ???, posted by linkadge on July 31, 2004, at 9:34:00
> Does anyone know this ??
>
> LinkadgeYes.
Acta Pharmacol Sin. 2000 Feb;21(2):151-5.
Bilobalide promotes expression of glial cell line-derived neurotrophic factor and vascular endothelial growth factor in rat astrocytes.
Zheng SX, Zhou LJ, Chen ZL, Yin ML, Zhu XZ.
Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 200031, China.
AIM: To study the effects of bilobalide on the expression of glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF) in rat astrocytes in vitro. METHODS: Semiquantification polymerase chain reaction (SQ-PCR) was used to investigate GDNF and VEGF mRNA expression in the astrocytes after bilobalide (5, 15, 50, 100 mumol.L-1) treatment. Immunohistochemistry method was used to detect GDNF and VEGF protein expression in cells treated with bilobalide 50 mumol.L-1 for 24 h. RESULTS: GDNF and VEGF mRNA increased markedly after astrocytes were treated with bilobalide 50 mumol.L-1 for 12 h. GDNF and VEGF protein were detected in the cytoplasm of astrocytes after the cells were treated with bilobalide 50 mumol.L-1 for 24 h. CONCLUSION: Bilobalide induced GDNF and VEGF expression in the cultured astrocytes.
poster:Larry Hoover
thread:372598
URL: http://www.dr-bob.org/babble/alter/20040718/msgs/372617.html